| Title |
Rqc2p and 60S ribosomal subunits mediate mRNA-independent elongation of nascent chains
|
|---|---|
| Published in |
Science, January 2015
|
| DOI | 10.1126/science.1259724 |
| Pubmed ID | |
| Authors |
Peter S Shen, Joseph Park, Yidan Qin, Xueming Li, Krishna Parsawar, Matthew H Larson, James Cox, Yifan Cheng, Alan M Lambowitz, Jonathan S Weissman, Onn Brandman, Adam Frost |
| Abstract |
In Eukarya, stalled translation induces 40S dissociation and recruitment of the ribosome quality control complex (RQC) to the 60S subunit, which mediates nascent chain degradation. Here we report cryo-electron microscopy structures revealing that the RQC components Rqc2p (YPL009C/Tae2) and Ltn1p (YMR247C/Rkr1) bind to the 60S subunit at sites exposed after 40S dissociation, placing the Ltn1p RING (Really Interesting New Gene) domain near the exit channel and Rqc2p over the P-site transfer RNA (tRNA). We further demonstrate that Rqc2p recruits alanine- and threonine-charged tRNA to the A site and directs the elongation of nascent chains independently of mRNA or 40S subunits. Our work uncovers an unexpected mechanism of protein synthesis, in which a protein--not an mRNA--determines tRNA recruitment and the tagging of nascent chains with carboxy-terminal Ala and Thr extensions ("CAT tails"). |
X Demographics
The data shown below were collected from the profiles of 86 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 22 | 26% |
| United Kingdom | 5 | 6% |
| Canada | 2 | 2% |
| France | 2 | 2% |
| Sweden | 1 | 1% |
| Latvia | 1 | 1% |
| Bulgaria | 1 | 1% |
| Poland | 1 | 1% |
| Spain | 1 | 1% |
| Other | 4 | 5% |
| Unknown | 46 | 53% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 53 | 62% |
| Scientists | 30 | 35% |
| Practitioners (doctors, other healthcare professionals) | 2 | 2% |
| Science communicators (journalists, bloggers, editors) | 1 | 1% |
Mendeley readers
The data shown below were compiled from readership statistics for 430 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 8 | 2% |
| United Kingdom | 4 | <1% |
| Germany | 2 | <1% |
| Spain | 2 | <1% |
| Italy | 1 | <1% |
| Sweden | 1 | <1% |
| South Africa | 1 | <1% |
| Cyprus | 1 | <1% |
| Canada | 1 | <1% |
| Other | 3 | <1% |
| Unknown | 406 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 135 | 31% |
| Researcher | 73 | 17% |
| Student > Bachelor | 43 | 10% |
| Student > Master | 37 | 9% |
| Student > Doctoral Student | 23 | 5% |
| Other | 57 | 13% |
| Unknown | 62 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 165 | 38% |
| Biochemistry, Genetics and Molecular Biology | 139 | 32% |
| Chemistry | 13 | 3% |
| Medicine and Dentistry | 11 | 3% |
| Neuroscience | 10 | 2% |
| Other | 21 | 5% |
| Unknown | 71 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 269. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 December 2022.
All research outputs
#135,158
of 25,522,520 outputs
Outputs from Science
#4,241
of 83,072 outputs
Outputs of similar age
#1,368
of 359,841 outputs
Outputs of similar age from Science
#66
of 1,064 outputs
Altmetric has tracked 25,522,520 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 83,072 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 65.8. This one has done particularly well, scoring higher than 94% of its peers.
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We're also able to compare this research output to 1,064 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 93% of its contemporaries.