@TonJeanne @MartinZ_uncut ça change pas qu'il a raison, un exemple : https://t.co/rmfyvAnnmT

@MathieuMolimard @SFPT_fr @univbordeaux @CHUdeLyon @CHUBordeaux @Sante_Gouv @ansm Il manque ça : https://t.co/HkVK6C9GKn

@QI_181 @JeremyAnso @JlsRet51206 @FrancoisFraiss1 @ThomSkell @xazalbert @FrLecardonnel @wargonm @france_soir Comme on peut le voir ici, l'HCQ n'a pas d'effet in vivo😅 https://t.co/e4x0mOrm8R https://t.co/XKe7UdHbk0

@zenbrainest @RothLabUNC It's intriguing that almost all of these molecules are phospholipidosis inducers. Something that perhaps we can look into @GestwickiLab @ShahmoraLab https://t.co/hIFG76kr4S

@QI_181 @Titithebest4 @raoult_didier Cette étude est très intéressante, mais si on regarde la source dans Science: https://t.co/4ORCI88lvz 1) Pourquoi ne pas avoir testé l'HCQ dans les expériences de la figure 5 sachant son accumulation dans les poumons à

@QI_181 Cette étude est très intéressante, mais si on regarde la source dans Science: https://t.co/4ORCI88lvz 1) Pourquoi ne pas avoir testé l'HCQ dans les expériences de la figure 5 sachant son accumulation dans les poumons à l'instar des molècules testée

@QI_181 @Titithebest4 Cette étude est très intéressante, mais si on regarde la source dans Science: https://t.co/QFe69FmDeI 1) Pourquoi ne pas avoir testé l'HCQ dans les expériences de la figure 5 sachant son accumulation dans les poumons à l'instar des mo

@exostuff_and_co @gillet_renaud @MartinZ_uncut Dommage, DARPA vous contredit : https://t.co/6r1GBQ1Wvv L'étude en question : https://t.co/mL3zIAYxMv Et la conclusion : https://t.co/hw5Nbpj4jx

@EricBillyFR @Locuste_ @SaiyanBio @lionel_case @Sonic_urticant bah ouai chez vous y'a de la bonne science de faite... y'a ça aussi mais chuuuut zizi va se ramener pour dire que c'est de la merde 🤣https://t.co/mVZTIOQRlA

RT @Navaraaa: @lionel_case @MartinZ_uncut @christophewyns @Petit_Louis1966 https://t.co/rmfyvAnnmT Hihi

@lionel_case @MartinZ_uncut @christophewyns @Petit_Louis1966 https://t.co/rmfyvAnnmT Hihi

@joerogan @elonmusk Here is a link that doesn’t look hackerish https://t.co/FfR4GsgDi8

Cuban is correct here generally…except on this. There is evidence that HCQ and Ivermectin are merely cell culture effects and that came out on June 22, 2021, coincidentally in Science when @BretWeinstein wanted an emergency @joerogan pod (#1671) b/c this h

@LockedFab @raoult_didier https://t.co/mVZTIOQRlA

@SahabiaAli @GUIGUI83457472 @RChabriet @roropapa4 @lebon80 @alpha_taury @sudokuvariante @LoLoWarn @ResilientsTv @olivesbts @Nodo23049099 @Martine88990455 @seraphus7 @insins31544458 @LePoint @IHU_Marseille @LerouxArthur10 @Pr_EChabriere @Olivierfente @tatia

@Khemtgat @winiwini65 @MatthieuMILLION @tatiann69922625 heu non c'est la réaction normal de quelqu'un qui a fait du développement thérapeutiques et donc des 10ene de manip par semaine in vitro avec aucune valeur translationnel in vivo... alors en clinique.

Et voilà pourquoi l'HCQ avait une efficacité In-Vitro ..... https://t.co/8XKlMhYRSM

@gstarp1 @antibetise @MahmoudZureik @SFPT_fr Je me suis renseigné contrairement à vous. https://t.co/fFDyCO2Ttm Unfortunately, drugs that have an antiviral effect in cells through phospholipidosis are unlikely to be effective in vivo.

@TonJeanne @AlaryPatrick @gmf09768505 @jjohana La source était dans l'article cité, pourtant. https://t.co/SKwyCoLIKk

@hobbyhorrible https://t.co/HXCCxOc6QL

RT @dronico: Drug-induced phospholipidosis confounds drug repurposing for SARS-CoV-2 Phospholipidosis is a shared mechanism underlying the…

Drug-induced phospholipidosis confounds drug repurposing for SARS-CoV-2 Phospholipidosis is a shared mechanism underlying the SARS-CoV-2 antiviral activity of many repurposed FIASMA drugs https://t.co/QPLwf8w443

@ErikGruchet @cavousf5 @CarolineFourest Et là l'article d'origine dans Science ⬇️ https://t.co/SKwyCoLIKk

@Stephan67358673 @JulienPain https://t.co/Y31zYjDAGi

#phospholipidosis https://t.co/PGHB7S4ZAv

@lionel_case Le truc énorme dans cette histoire c est que leur argument de Sigma récepteur a été démontré comme faux dans une magnifique papier dans Science https://t.co/Y31zYjDAGi

Article intéressant qui expliquerai l'efficacité in vitro de certains médicaments contre la #COVID19 vs in Vivo. https://t.co/BYC27K7aYM

A good time to bring this paper to attention, explaining a lot of misunderstood in vitro results in #COVID19 screens - https://t.co/SUP3rPxd16

@Tsogoeva_Group @HartungIngo Svetlana, we can't rule this out. I think to exploit DIPL therapeutically we'd want to understand molecular mechanism. Testing 5 DIPLs for anti-viral effects in animals, Garcia-Sastre lab saw no effect other than apparent toxic

RT @HartungIngo: A bit unfortunate to see the Shoichet study ignored in this publication about SARS-CoV-2 leads. Through the eyes of a medi…

RT @HartungIngo: A bit unfortunate to see the Shoichet study ignored in this publication about SARS-CoV-2 leads. Through the eyes of a medi…

RT @HartungIngo: A bit unfortunate to see the Shoichet study ignored in this publication about SARS-CoV-2 leads. Through the eyes of a medi…

A bit unfortunate to see the Shoichet study ignored in this publication about SARS-CoV-2 leads. Through the eyes of a medicinal chemist the significance seems obvious: https://t.co/9Ag6vcSKvq @BShoichet

RT @UcsfPspg: Student Spotlight: Congratulations to Tia Tummino on her publication! https://t.co/DfUyAqvAn6

Student Spotlight: Congratulations to Tia Tummino on her publication! https://t.co/DfUyAqvAn6

@canardbruno @flodebarre @DecrolyE https://t.co/Y31zYjDAGi

RT @BShoichet: @HartungIngo @jchodera @foldingathome @covid_moonshot DIPL is common among SARS-2 antivirals--and others!-- and is a big was…

There are no 100% perfect control experiments, but I think that being able to generate structure-activity relationships is the best safety net we have. Is activity driven by structural features or mere chemical properties? https://t.co/VYHHJXvgxO https://t

RT @BShoichet: @HartungIngo @jchodera @foldingathome @covid_moonshot DIPL is common among SARS-2 antivirals--and others!-- and is a big was…

RT @BShoichet: @HartungIngo @jchodera @foldingathome @covid_moonshot DIPL is common among SARS-2 antivirals--and others!-- and is a big was…

@HartungIngo @jchodera @foldingathome @covid_moonshot DIPL is common among SARS-2 antivirals--and others!-- and is a big waste of effort (our own experience was painful). With the Pognan, Moretti and @VignuzziLab, we published on how to detect it https://t

https://t.co/crLwYU0jCi

@trailrunner402 @dimlabim @farid__jalali @HoertelN @EricLenze2 See this paper if you want more explanation about phospholipidosis. It may be mainly relevant as a confounder, but it might also have some relevant antiviral action, perhaps especially if a dru

@NoyesJHumphrey @farid__jalali @dimlabim @HoertelN @EricLenze2 https://t.co/crLwYU0jCi

RT @DARPA: Researchers working on DARPA's PANACEA program have identified a mechanism that may help explain why many repurposed drugs (such…

@andybliu Not yet clear if Benadryl helps. Would need to be tested in a RCT first. Some drugs seems to work in the lab, but don’t turn out to be effective in people. For example, see this article: https://t.co/YM6i7PQSbX

I’d like to know how many of these drugs also induce phospholipidosis and whether that explains any of the apparent antiviral effects. https://t.co/YM6i7PQSbX

@PUAM_info @BombingBelgium Si c'est libération qui vous gêne, voilà l'article scientifique à la base de cet article https://t.co/8XKlMhYRSM

@UToc2Me @nqtv https://t.co/s2ARauEKlZ Ouais tu as raison un médicament agis sur des éléments et le virus sur d'autre, c'est bien de choisir un médicament qui agis sur les mêmes éléments que le virus, sinon sa ne sert à rien https://t.co/nzqVG85dHK

RT @chunjp: @inferist ありがとうございます。 https://t.co/RWjAy5fnDz ですね。まさにこういう死亡例(この場合はアッセイ系の罠で死亡)をコレクションしたいみたいな意図です。

@inferist ありがとうございます。 https://t.co/RWjAy5fnDz ですね。まさにこういう死亡例(この場合はアッセイ系の罠で死亡)をコレクションしたいみたいな意図です。

@Majormac1 @NEJM This is one reason, why cell culture experiments are misleading. I am also not arguing. I am just saying that it is not as clear as many want us to believe. There is too much ideology in the discussions, and it is complicated. https://t.c

RT @drnic1: The #science is clear that HCT therapy is ineffective in #COVID19 This paper dos a good job of explaining why in vitro testing…

RT @alfobioq: Why pharmacology is important when a biologist enters to drug discovery field!! Drug-induced phospholipidosis confounds drug…

RT @emulenews: #ScienceMag Drug-induced phospholipidosis confounds drug repurposing for SARS-CoV-2 https://t.co/9ZwToVr6dD There is a stron…

RT @VignuzziLab: Our collaboration with @BShoichet lab @QBI_UCSF and @NovartisScience on our/the troubles with drug repurposing now out in…

RT @lipidchronicles: Drug-induced phospholipidosis confounds drug repurposing for SARS-CoV-2 https://t.co/1WF79kkQJb (@ScienceMagazine) htt…

RT @roby_bhatt: Fascinating paper (https://t.co/cZc6detzwW) & commentary (https://t.co/XncZUPp7Pk) on a common mechanism of confounding for…

@farid__jalali @ENirenberg @Boghuma …antiviral effect, regulate coagulopathy or mitigate cytokine storm, which are known hallmarks of severe COVID-19." Sounds pretty slippery to me… esp since the interference with endolysosomal viral trafficking is probab

@zoonotic1 @ENirenberg @Boghuma Especially after this paper on how apparent antiviral effects of sigma receptor ligands are probably artifactual… one of my legit favorite papers of the pandemic: https://t.co/A3nSx7jvqE https://t.co/KcStyLlugI

@EwenLescop Cool results, & much hard work! I wonder if phospholipidosis played a role in the original study? (https://t.co/hIFG762PGi)Sertraline is a phospholipidosis inducer, and thioridizine likely is. Another ding on mechanism-free repurposing? @z

@Iwantoffearth @culaterlater @MandelaFace @sunluvver @KCroakeHeisler To clarify, high concentrations of drugs can appear to be effective in vitro because of confounding phospholipidosis effects, so it still hasn't actually been shown to be effective! https

@GidMK In vitro studies have failed in the clinic for decades. This might be another reason https://t.co/iKdvtaaRso

RT @souyakuchan: Ethoxzolamide: logP 2.01, pKa 8.0 https://t.co/8SjlNmED4V こういう物性の薬は特異的に効いているわけではなくvivoでは効かないという話 (https://t.co/YEQ0J3kRpZ…

RT @souyakuchan: Ethoxzolamide: logP 2.01, pKa 8.0 https://t.co/8SjlNmED4V こういう物性の薬は特異的に効いているわけではなくvivoでは効かないという話 (https://t.co/YEQ0J3kRpZ…

Ethoxzolamide: logP 2.01, pKa 8.0 https://t.co/8SjlNmED4V こういう物性の薬は特異的に効いているわけではなくvivoでは効かないという話 (https://t.co/YEQ0J3kRpZ) もあればそうとも言い切れないという話 (https://t.co/xcmSWR1fo6) もある。査読で言われるだろうが一定の検証はしてほしいところ

@HackerVol @mmpadellan light work? this isn't some culture war bullshit, this about medical efficacy. HQC was proven to only appear to have anti viral activity b/c it induces phospholipidosis, which is harmful. https://t.co/OXMH00sUIK

RT @chupvl: Basically, whenever we have a bioact.compound with cationic amphiphilic nature (cLogP >= 3, pKa >= 7.4) and its activity >100nM…

Basically, whenever we have a bioact.compound with cationic amphiphilic nature (cLogP >= 3, pKa >= 7.4) and its activity >100nM we should double-check for phospholipidosis. I think it's valid for almost any drug program https://t.co/PvSUA3w7XX

RT @JPottel: A great thread if you want an example of how to communicate politely and poignantly in scientific discourse. Very glad to have…

RT @BShoichet: @zenbrainest Ok, ok, if you insist. This paper argues that some CADs will not induce phospholipidosis (PLD) & can be product…

RT @BShoichet: @zenbrainest Ok, ok, if you insist. This paper argues that some CADs will not induce phospholipidosis (PLD) & can be product…

RT @JPottel: A great thread if you want an example of how to communicate politely and poignantly in scientific discourse. Very glad to have…

A great thread if you want an example of how to communicate politely and poignantly in scientific discourse. Very glad to have spent a few years with Brian learning some of this first-hand. #ChemTwitter

@zenbrainest Ok, ok, if you insist. This paper argues that some CADs will not induce phospholipidosis (PLD) & can be productively antiviral. No argument! This was not the point of https://t.co/hIFG762PGi which was instead that when antiviral IC50 super

RT @Olive_Free: @VidalFrederique 6 milliards de $ en pure perte pour des essais d'efficacité in vivo de l'hydroxychloroquine qui n'avaient…

RT @Olive_Free: @VidalFrederique 6 milliards de $ en pure perte pour des essais d'efficacité in vivo de l'hydroxychloroquine qui n'avaient…

@VidalFrederique 6 milliards de $ en pure perte pour des essais d'efficacité in vivo de l'hydroxychloroquine qui n'avaient aucune chance d'aboutir: bravo, quel succès de la science française grâce au soutien politique dont a bénéficié Didier Raoult!👏 Cocor

@nossolar3 @CaudeHenrion @raoult_didier @PrJoyeux Il ne s'agit pas d'idées mais de faits démontrés. Ainsi fonctionne la science. Je vous ai déjà proposé de lire l'article suivant pour comprendre pourquoi certaines molécules fonctionnent in vitro mais pas i

@nossolar3 @Yoda552 @RositaFavia L'article de référence dans Science : https://t.co/csqd8dyEXU

RT @Locuste_: Intuition confirmée au terme de leur étude publiée dans Science : l'effet in vitro de ces molécules repose bel et bien sur un…

Intuition confirmée au terme de leur étude publiée dans Science : l'effet in vitro de ces molécules repose bel et bien sur une propriété partagée : elles induisent un phénomène appelé phospholipidose, qui se distingue de l'action antivirale. https://t.co/

Drug-induced phospholipidosis confounds drug repurposing for SARS-CoV-2 https://t.co/4XCsH2d2gB Important study illustrating failure of repurposing drugs based on hypothesis-free screen approach. Also shows risk of relying on cell-based assay measuring a d

Original work: Drug-induced phospholipidosis confounds drug repurposing for SARS-CoV-2 https://t.co/67Qhd3patx

RT @souyakuchan: リポジショニングでコロナに効きそうとされ(て結局だめだっ)た薬の多くは大体カチオン性両親媒性薬でホスホリピドーシスの惹起によって見かけ上効いていたのではという話 https://t.co/ysX1K6WaeU

RT @lipidchronicles: Drug-induced phospholipidosis confounds drug repurposing for SARS-CoV-2 https://t.co/1WF79kkQJb (@ScienceMagazine) htt…

RT @lipidchronicles: Drug-induced phospholipidosis confounds drug repurposing for SARS-CoV-2 https://t.co/1WF79kkQJb (@ScienceMagazine) htt…

Drug-induced phospholipidosis confounds drug repurposing for SARS-CoV-2 https://t.co/1WF79kkQJb (@ScienceMagazine) https://t.co/P0NDCzUqxR

RT @BShoichet: This paper now out in Science https://t.co/4TQBSx6Mml. Key observations: (1/N)

@Covid19Crusher A frontline research group !. Yes, an excellent overview of the potential of FIASMAs on COVID-19. However, inhibition of ASM correlated with drug-induced PLD, and this is related to its antiviral activity. An open question https://t.co/c1og

@ENirenberg @GidMK Need to look over this latest study carefully; I agree prior studies were tiny but this one was randomized & larger, right? My biggest pause is the hand-wavy mechanism of action - I thought sigma-1 receptors were a false lead (https:

@CyrilPedia @Merz From the structure, it looks like a like a prime false hit for phospholipidosis induced blocking of viral replication that wouldn't work in people. Good Science article here https://t.co/5y4Qz0L9Ch

RT @marco_heffler: Link paper: https://t.co/CsCVzSvOeq Link riassunto su Science: https://t.co/XBeUM2A4xw 10/11

RT @marco_heffler: Link paper: https://t.co/CsCVzSvOeq Link riassunto su Science: https://t.co/XBeUM2A4xw 10/11

Molto interessante 👏

RT @marco_heffler: Link paper: https://t.co/CsCVzSvOeq Link riassunto su Science: https://t.co/XBeUM2A4xw 10/11

RT @marco_heffler: Link paper: https://t.co/CsCVzSvOeq Link riassunto su Science: https://t.co/XBeUM2A4xw 10/11

RT @marco_heffler: Link paper: https://t.co/CsCVzSvOeq Link riassunto su Science: https://t.co/XBeUM2A4xw 10/11

RT @marco_heffler: Link paper: https://t.co/CsCVzSvOeq Link riassunto su Science: https://t.co/XBeUM2A4xw 10/11

RT @marco_heffler: Link paper: https://t.co/CsCVzSvOeq Link riassunto su Science: https://t.co/XBeUM2A4xw 10/11