| Title |
The cytotoxic Staphylococcus aureus PSMα3 reveals a cross-α amyloid-like fibril
|
|---|---|
| Published in |
Science, February 2017
|
| DOI | 10.1126/science.aaf4901 |
| Pubmed ID | |
| Authors |
Einav Tayeb-Fligelman, Orly Tabachnikov, Asher Moshe, Orit Goldshmidt-Tran, Michael R Sawaya, Nicolas Coquelle, Jacques-Philippe Colletier, Meytal Landau |
| Abstract |
Amyloids are ordered protein aggregates, found in all kingdoms of life, and are involved in aggregation diseases as well as in physiological activities. In microbes, functional amyloids are often key virulence determinants, yet the structural basis for their activity remains elusive. We determined the fibril structure and function of the highly toxic, 22-residue phenol-soluble modulin α3 (PSMα3) peptide secreted by Staphylococcus aureus PSMα3 formed elongated fibrils that shared the morphological and tinctorial characteristics of canonical cross-β eukaryotic amyloids. However, the crystal structure of full-length PSMα3, solved de novo at 1.45 angstrom resolution, revealed a distinctive "cross-α" amyloid-like architecture, in which amphipathic α helices stacked perpendicular to the fibril axis into tight self-associating sheets. The cross-α fibrillation of PSMα3 facilitated cytotoxicity, suggesting that this assembly mode underlies function in S. aureus. |
X Demographics
The data shown below were collected from the profiles of 69 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 13 | 19% |
| United Kingdom | 4 | 6% |
| France | 3 | 4% |
| Switzerland | 2 | 3% |
| Italy | 2 | 3% |
| Finland | 1 | 1% |
| Central African Republic | 1 | 1% |
| Brazil | 1 | 1% |
| Spain | 1 | 1% |
| Other | 4 | 6% |
| Unknown | 37 | 54% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 46 | 67% |
| Scientists | 22 | 32% |
| Practitioners (doctors, other healthcare professionals) | 1 | 1% |
Mendeley readers
The data shown below were compiled from readership statistics for 264 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | <1% |
| China | 1 | <1% |
| Unknown | 262 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 60 | 23% |
| Researcher | 42 | 16% |
| Student > Bachelor | 25 | 9% |
| Student > Master | 20 | 8% |
| Student > Doctoral Student | 15 | 6% |
| Other | 43 | 16% |
| Unknown | 59 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 62 | 23% |
| Chemistry | 41 | 16% |
| Agricultural and Biological Sciences | 31 | 12% |
| Engineering | 15 | 6% |
| Unspecified | 14 | 5% |
| Other | 33 | 13% |
| Unknown | 68 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 119. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 November 2023.
All research outputs
#345,735
of 25,210,618 outputs
Outputs from Science
#8,786
of 80,694 outputs
Outputs of similar age
#7,357
of 317,906 outputs
Outputs of similar age from Science
#155
of 1,048 outputs
Altmetric has tracked 25,210,618 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 98th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 80,694 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 65.3. This one has done well, scoring higher than 89% of its peers.
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We're also able to compare this research output to 1,048 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 85% of its contemporaries.